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pgl-3 promoter plasmids  (Promega)

 
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    Promega pgl-3 promoter plasmids
    Pgl 3 Promoter Plasmids, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgl-3+promoter+plasmids/pm33165812-390-7-10?v=Promega
    Average 90 stars, based on 1 article reviews
    pgl-3 promoter plasmids - by Bioz Stars, 2026-07
    90/100 stars

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    Expression of NLRP3 in PC tissues and cell lines, and association between NLRP3 and XLOC_000647. a Representative images (×100 and ×400) of H and E (h ematoxylin and eosin) and immunohistochemical (IHC) staining for NLRP3 in paraffin-embedded PC and corresponding adjacent tissues. b Relative XLOC_00067 expression in tissues ( n = 48). PC tissues versus corresponding adjacent tissues. c Relative XLOC_000647 expression in cell lines and the mean ± SD from three independent experiments. d Influence of XLOC_000647-stable overexpression on the expression level of NLRP3 in cell lines by western blot. e Representative images (×100 and ×400) of IHC staining of the tumor from mice. Results showed that overexpression of XLOC_000647 decreased the expression level of NLRP3. f The correlation between NLRP3 mRNA levels and XLOC_00067 levels in 48 PC tissues (R 2 = 0.4407, P < 0.001). g The <t>luciferase</t> activity of NLRP3 promoter is decreased by XLOC_000647 in 293 T cells. NS (not significant). *** P < 0.001
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    Expression of NLRP3 in PC tissues and cell lines, and association between NLRP3 and XLOC_000647. a Representative images (×100 and ×400) of H and E (h ematoxylin and eosin) and immunohistochemical (IHC) staining for NLRP3 in paraffin-embedded PC and corresponding adjacent tissues. b Relative XLOC_00067 expression in tissues ( n = 48). PC tissues versus corresponding adjacent tissues. c Relative XLOC_000647 expression in cell lines and the mean ± SD from three independent experiments. d Influence of XLOC_000647-stable overexpression on the expression level of NLRP3 in cell lines by western blot. e Representative images (×100 and ×400) of IHC staining of the tumor from mice. Results showed that overexpression of XLOC_000647 decreased the expression level of NLRP3. f The correlation between NLRP3 mRNA levels and XLOC_00067 levels in 48 PC tissues (R 2 = 0.4407, P < 0.001). g The <t>luciferase</t> activity of NLRP3 promoter is decreased by XLOC_000647 in 293 T cells. NS (not significant). *** P < 0.001
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    Image Search Results


    Expression of NLRP3 in PC tissues and cell lines, and association between NLRP3 and XLOC_000647. a Representative images (×100 and ×400) of H and E (h ematoxylin and eosin) and immunohistochemical (IHC) staining for NLRP3 in paraffin-embedded PC and corresponding adjacent tissues. b Relative XLOC_00067 expression in tissues ( n = 48). PC tissues versus corresponding adjacent tissues. c Relative XLOC_000647 expression in cell lines and the mean ± SD from three independent experiments. d Influence of XLOC_000647-stable overexpression on the expression level of NLRP3 in cell lines by western blot. e Representative images (×100 and ×400) of IHC staining of the tumor from mice. Results showed that overexpression of XLOC_000647 decreased the expression level of NLRP3. f The correlation between NLRP3 mRNA levels and XLOC_00067 levels in 48 PC tissues (R 2 = 0.4407, P < 0.001). g The luciferase activity of NLRP3 promoter is decreased by XLOC_000647 in 293 T cells. NS (not significant). *** P < 0.001

    Journal: Molecular Cancer

    Article Title: Long non-coding RNA XLOC_000647 suppresses progression of pancreatic cancer and decreases epithelial-mesenchymal transition-induced cell invasion by down-regulating NLRP3

    doi: 10.1186/s12943-018-0761-9

    Figure Lengend Snippet: Expression of NLRP3 in PC tissues and cell lines, and association between NLRP3 and XLOC_000647. a Representative images (×100 and ×400) of H and E (h ematoxylin and eosin) and immunohistochemical (IHC) staining for NLRP3 in paraffin-embedded PC and corresponding adjacent tissues. b Relative XLOC_00067 expression in tissues ( n = 48). PC tissues versus corresponding adjacent tissues. c Relative XLOC_000647 expression in cell lines and the mean ± SD from three independent experiments. d Influence of XLOC_000647-stable overexpression on the expression level of NLRP3 in cell lines by western blot. e Representative images (×100 and ×400) of IHC staining of the tumor from mice. Results showed that overexpression of XLOC_000647 decreased the expression level of NLRP3. f The correlation between NLRP3 mRNA levels and XLOC_00067 levels in 48 PC tissues (R 2 = 0.4407, P < 0.001). g The luciferase activity of NLRP3 promoter is decreased by XLOC_000647 in 293 T cells. NS (not significant). *** P < 0.001

    Article Snippet: Luciferase reporter plasmid containing NLRP3 promoter sequence (NLRP3 promoter-pGL-3-Basic, referred to as NLRP3 promoter) and its corresponding negative control pGL-3-Basic were purchased from Vigene Biosciences (Shandong, China).

    Techniques: Expressing, Immunohistochemical staining, Immunohistochemistry, Over Expression, Western Blot, Luciferase, Activity Assay